KMID : 0854720080280030220
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Korean Journal of Asthma, Allergy and Clinical Immunology 2008 Volume.28 No. 3 p.220 ~ p.225
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Effects of GR¥â Overexpression on Steroid-induced Changes of the Whole Genes in the A549 Airway Epithelial Cells
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Lee Ho-Yeon
Park Jae-Seuk Jee Young-Koo Kim Youn-Seup Kim Do-Hyung Kim Nam-Hee Kim Dong-Hoon
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Abstract
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Background: Glucocorticoid receptor ¥â (GR¥â) may not bind to ligands and is thought to affect gene transcription only by acting as a dominant negative inhibitor of Glucocorticoid receptor ¥á (GR¥á). However, there have been few reports about the functional roles of GR¥â in the steroid-induces changes of gene expression.
Objective: To investigate the functional role of GR¥â overexpression in the steroid- induced changes of gene expression.
Method: The changes in gene expressions such as steroidinduced synthesis of genes, TNF-¥á-induced synthesis of inflammatory genes and steroid induced suppression of activated genes by TNF-¥á treatment were compared with and without pCMV GR¥â expression.
Result: The number of genes increased by dexamethasone treatment only in the GR¥â transfected group were 54.
Increased expressions of genes by dexamethasone treatment were not affected by GR¥â overexpression. The number of
genes increased by TNF-¥á treatment only in the GR¥â -transfected group were 69. Expressions of genes increased by TNF-¥á treatment in both the control and the GR¥â -transfected group were decreased by further dexamethasone treatment in a similar manner.
Conclusion:The results of this study suggest that GR¥â may not be a dominant negative inhibitor in the teroidinduced changes of genes and that it may have some functional roles in the regulation of gene expressions.
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KEYWORD
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Glucocorticoid receptor ¥â, Steroid
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